By Thomas Dieing, Olaf Hollricher, Jan Toporski
Confocal Raman Microscopy is a comparatively new process that enables chemical imaging with no particular pattern guidance. via integrating a delicate Raman spectrometer inside a state of the art microscope, Raman microscopy with a spatial answer all the way down to 200nm laterally and 500nm vertically should be accomplished utilizing seen mild excitation. fresh advancements in detector and machine know-how in addition to optimized tool layout have diminished integration instances of Raman spectra via orders of value, in order that whole photos inclusive of tens of millions of Raman spectra should be bought in seconds or mins instead of hours, which was normal only one decade in the past. the aim of this e-book is to supply the reader a complete evaluate of the speedily constructing box of Confocal Raman Microscopy and its applications.
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Additional info for Confocal Raman Microscopy
In case resonance Raman spectroscopy occurs via the same electronic excited states where the fluorescence emission originates (Fig. 4a), the Raman signals are often masked by the broad and featureless fluorescence spectra. , the energy difference between the absorption maximum and the fluorescence maximum, is only small (Fig. 4c). In UV resonance Raman spectroscopy, energy-rich UV light is used, so that higher electronic excited states are incorporated. Raman scattering occurs within the first few femtoseconds (10−15 s) directly from the high electronic state.
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The imaging of simple objects (Academic, London, 1990) pp. 25–30 7. T. Wilson, Confocal Microscopy, Chap. 2. The imaging of simple objects (Academic, London, 1990) pp. 41–45 8. T. R. Carlini, Opt. Lett. 12, 227 (April 1987) 9. R. Sheppard, M. Gu, Optik 86(3), 104 (1990) Chapter 2 Introduction to the Fundamentals of Raman Spectroscopy Benjamin Dietzek, Dana Cialla, Michael Schmitt, and Jürgen Popp Abstract The goal of this chapter is to provide a general introduction to the principles of Raman spectroscopy.
Confocal Raman Microscopy by Thomas Dieing, Olaf Hollricher, Jan Toporski